Recombinant microorganisms with mixed sugar utilization
AssigneeThe Board of Trustees of the University of Illinois
InventorsYong-Su Jin, Stephan Thomas Lane, Haiqing Xu, Soo-Rin Kim
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Abstract
The present disclosure provides recombinant microorganisms capable of sugar co-utilization, the recombinant microorganism comprising a genetically altered microorganism (e.g. S. cerevisiae) having a lack of, or reduced expression of, or expression of truncated or mutated forms of at least one polypeptide selected from Hxk1, Hxk2, and Glk1. Reduction of the expression or biological activity of Hxk1, Hxk2 and Glk1 leads to reduction in the glucose consumption pathway, allowing the microorganism to co-utilize multiple sugars (e.g. glucose, xylose, galactose) at an improved rate.
Claims26
A genetically engineered yeast having attenuated expression, as compared to a wild-type or control yeast, of a polynucleotide encoding:
- a hexokinase isoenzyme 1 (“Hxk1”) polypeptide;
- a hexokinase isoenzyme 2 (“Hxk2”) polypeptide and a glucokinase-1 (“Glk1”) polypeptide;
- a Glk1 polypeptide;
- a Hxk1 polypeptide and a Hxk2 polypeptide;
- a Glk1 polypeptide and a Hxk1 polypeptide;
- or a Hxk1 polypeptide, a Hxk2 polypeptide, and a Glk1 polypeptide.
A genetically engineered yeast having an ability to co-utilize sugars, wherein the biological activity of an endogenous protein having at least 90% sequence identity to an amino acid sequence set forth in:
- SEQ ID NO:74;
- SEQ ID NO:74 and 76;
- SEQ ID NO:74, 76, and 78;
- SEQ ID NO:78;
- SEQ ID NO:76 and 78;
- or SEQ ID NO:74 and 78 is reduced as compared to a control yeast.
A genetically engineered yeast comprising a polynucleotide encoding at least one mutant polypeptide selected from Hxk1 T89A, Hxk2 P455F, and Glk1 S306P.
Dependencies are inferred from the claim text.