Method for sequencing oligosaccharides
AssigneesCentre National De La Recherche Scientifique, Universite Claude Bernard Lyon 1
InventorsIsabelle Compagnon, Baptiste Schindler
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Abstract
The present invention concerns a method for sequencing oligosaccharides, which makes it possible to identify the primary sequence of an oligosaccharide of unknown structure, including its monosaccharide composition, the position (regiochemistry) and configuration (stereochemistry) of glycosidic bonds, the nature and position of functional modifications, and its branched structure, particularly including the identification of the reducing end.
Claims8
A method for sequencing oligosaccharides, wherein it comprises the steps of
- i. fragmentation of the oligosaccharides into disaccharides and monosaccharides while preserving the molecular structure of the constituents as present in the oligosaccharide to be sequenced
- ii. separation of each previously obtained disaccharide and monosaccharide by mass spectrometry,
- iii. analysis by infrared (IR) vibrational spectroscopy of each previously separated disaccharide and monosaccharide,
- iv. identification of the structure of each disaccharide and monosaccharide by comparison of the obtained IR spectra with a set of reference disaccharide and monosaccharide IR spectra, and
- v. definition of the oligosaccharide sequence by combination of the structures identified for each disaccharide and monosaccharide.
An apparatus for sequencing oligosaccharides comprising a mass spectrometry device, an electromagnetic radiation source, a database and processing means, wherein it comprises a processor for controlling the steps of
- i. fragmentation of the oligosaccharides into disaccharides and monosaccharides while preserving the molecular structure of the constituents as present in the oligosaccharide to be sequenced
- ii. separation of each previously obtained disaccharide and monosaccharide by mass spectrometry with the mass spectrometry device,
- iii. analysis by infrared (IR) vibrational spectroscopy of each previously separated disaccharide and monosaccharide with the electromagnetic radiation source,
- iv. identification of the structure of each disaccharide and monosaccharide by comparison of the obtained IR spectra with a set of reference disaccharide and monosaccharide IR spectra contained in the database, and
- v. definition of the oligosaccharide sequence by combination of the structures identified for each disaccharide and monosaccharide by the processing means.
Dependencies are inferred from the claim text.