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US 10,832,404 B2Granted
Automated detection and repositioning of micro-objects in microfluidic devices
AssigneeBerkeley Lights, Inc.
InventorsFenglei Du, Paul M. Lundquist, John A. Tenney, Troy A. Lionberger
Life of this patent
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Abstract
Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.
Claims30
A method of re-positioning a micro-object in a microfluidic device comprising a plurality of sequestration pens, the method comprising:
- identifying a first micro-object of a set of micro-objects disposed within the microfluidic device;
- computing one or more trajectories for the first micro-object, each trajectory comprising a path connecting the first micro-object, at a first location with a second location within the microfluidic device, wherein the first or second location is located in a sequestration pen of the plurality of sequestration pens disposed within the microfluidic device;
- selecting a trajectory from the one or more trajectories computed for the first micro-object, wherein the length of the selected trajectory is minimized;
- and
- re-positioning the micro-object by moving the micro-object along its selected trajectory.
2 more elements
A method of re-positioning a first micro-object in a microfluidic device comprising a plurality of sequestration pens, the method comprising:
- identifying a first micro-object disposed within the microfluidic device;
- computing one or more trajectories for the first micro-object, each trajectory of the one or more trajectories comprising a path connecting the first micro-object at a first location with a second location within the microfluidic device, wherein the first or second location is located in a sequestration pen of the plurality of sequestration pens;
- selecting a trajectory from the one or more trajectories for the first micro-object by determining whether the trajectory exceeds a pre-determined maximum length;
- and
- re-positioning the first micro-object by moving the first micro-object along its selected trajectory.
2 more elements
Dependencies are inferred from the claim text.