Transformant that produces copolymerized PHA containing 3HH unit, and method for producing said PHA
AssigneeKaneka Corporation
InventorsHisashi Arikawa, Keiji Matsumoto
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Abstract
Provided are a transformant that produces a copolymerized PHA containing 3HH units in a higher composition proportion; and a method for producing a copolymerized PHA, using this transformant. The transformant is a transformant that produces a copolymerized PHA containing 3HH units, in which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced into a prokaryotic microorganism having a PHA synthetase gene capable of synthesizing the copolymerized PHA containing the 3HH units. The method is a method for producing a copolymerized PHA containing 3HH units, which includes a step of culturing this transformant.
Claims18
A transformant that produces a copolymerized polyhydroxyalkanoate (PHA) comprising a 3-hydroxyhexanoic acid (3HH) unit, wherein the transformant is a prokaryotic microorganism into which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced, wherein the prokaryotic microorganism comprises a PHA synthetase gene capable of synthesizing the copolymerized PHA comprising the 3HH units, and the gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is derived from an eukaryote,
- wherein the prokaryotic microorganism is Cupriavidus necator, and
- wherein the gene encoding the enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is a gene derived from Yarrowia lipolytica.
A transformant that produces a copolymerized polyhydroxyalkanoate (PHA) comprising a 3-hydroxyhexanoic acid (3HH) unit, wherein the transformant is a prokaryotic microorganism into which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced, wherein the prokaryotic microorganism comprises a PHA synthetase gene capable of synthesizing the copolymerized PHA comprising the 3HH units, and the gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is derived from an eukaryote,
- wherein the prokaryotic microorganism is Cupriavidus necator, and
- wherein the gene encoding the enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is a gene derived from Drosophila melanogaster.
Dependencies are inferred from the claim text.