# US 10,829,785 B2 · Nucleic acid application primers

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- Number: US 10,829,785 B2
- Document ID: US10829785B2
- Status: Granted
- Kind: B2
- Application number: 16/681,964
- Filed: 2019-11-13
- Published: 2020-03-05
- Granted: 2020-11-10
- Earliest claimed: 2007-02-12
- Assignees: Trizell Ltd.
- Applicants: Trizell Ltd.
- Inventors: Hanna P. Lesch; Kari J. Airenne; Seppo Yla-Herttuala
- Web page: https://patentgrep.com/patent/US10829785B2

## CPC

- C12N 15/86: Viral vectors
- A61K 48/0091: {Purification or manufacturing processes for gene therapy compositions}
- C07K 14/005: from viruses
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C12N 2710/14021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2710/14043: viral genome or elements thereof as genetic vectore
- C12N 2710/14051: Methods of production or purification of viral material
- C12N 2740/15043: viral genome or elements thereof as genetic vector
- C12N 2740/15051: Methods of production or purification of viral material
- C12N 2740/16022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2740/16043: viral genome or elements thereof as genetic vector
- C12N 2740/16045: Special targeting system for viral vectors
- C12N 2740/16051: Methods of production or purification of viral material
- C12N 2810/6081: rhabdoviridae, e.g. VSV
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Novel forward primer, reverse primer and poly-linker suitable for replication of nucleic acids in e.g., 293 cells.

## Claims

### Claim 1

A DNA having a sequence selected from SEQ ID No: 1; SEQ ID No: 2; or SEQ ID No: 3.

### Claim 2 (depends on 1)

The DNA of claim 1, the DNA having the sequence of SEQ ID No: 1.

### Claim 3 (depends on 1)

The DNA of claim 1, the DNA having the sequence of SEQ ID No: 2.

### Claim 4 (depends on 1)

The DNA of claim 1, the DNA having the sequence of SEQ ID No: 3.