Mutant lipase and use thereof

A method for generating a variant polypeptide having lipase activity wherein the method comprises

• a. selecting a parent polypeptide comprising at least 80% identity to the amino acid sequence according to SEQ ID NO:
• 1;
• and,
• b. substituting at least one amino acid into Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534, wherein said position is defined with reference to SEQ ID NO:
• 1, wherein alanine at position 1 in SEQ ID NO:
• 1 is counted as number 1;
• and
• c. generating the variant polypeptide, wherein the polypeptide having lipase activity has a higher specificity towards butyrate than the specificity towards octanoate and/or palmitate.

A nucleic acid encoding a lipase, which has at least 80% sequence identity to SEQ ID NO:

• 2, wherein SEQ ID NO:
• 2 comprises at least one mutation resulting in Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534 of the amino acid sequence of SEQ ID NO:
• 1, wherein the position is defined with reference to SEQ ID NO:
• 1, wherein alanine at position 1 in SEQ ID NO:
• 1 is counted as number 1.