Production of xylitol from glucose by a recombinant strain

A method for producing xylitol, the method comprising:

• culturing in a culture medium a recombinant Pichia ohmeri capable of producing a xylitol titer of at least 15 g/L, in a supernatant within 48 h of culture, the recombinant Pichia ohmeri comprising:
• a heterologous nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) from Escherichia coli and/or Ralstonia solanacearum comprising the amino acid sequence of SEQ ID NO:
• 2 and/or 43 using D-arabitol as a substrate and producing D-xylulose as a product;
• and
• a heterologous nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase from Pichia stipitis and/or Gluconobacter oxydans comprising the amino acid sequence of SEQ ID NO:
• 5 and/or 8 using D-xylulose as a substrate and producing xylitol as a product;
• and
• recovering the produced xylitol.