Point-of-care and/or portable platform for gene therapy

A method performed by a device, the method comprising:

• receiving, by a treatment chamber, a subject sample comprising at least one first cell type and at least one second cell type;
• transferring the subject sample from the treatment chamber to at least one cell type separator;
• separating the at least one first cell type in the subject sample by allowing the at least one second cell type to pass through the cell type separator and retaining the at least one first cell type within the at least one cell type separator;
• transferring the at least one first cell type into the treatment chamber;
• introducing a genetic modifier to the at least one first cell type to generate genetically-modified cells;
• pelleting the genetically-modified cells within the treatment chamber through centrifugation to create a pelleted cell suspension;
• removing a specified supernatant volume from the pelleted cell suspension through at least one first conduit connected to the treatment chamber;
• diluting the pelleted cell suspension with media comprising a pharmaceutically acceptable carrier;
• centrifuging the diluted pelleted cell suspension and removing an additional volume of supernatant in the treatment chamber through the at least one first conduit to form a final cell product formulation;
• and
• transferring the final cell product formulation into one or more sterile receptacles through a second conduit connected to the treatment chamber wherein the final cell product formulation is ready for administration to a subject upon completion of transfer into the one or more sterile receptacles.