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US 10,822,634 B2Granted
Determination of ligninases activities by nano-structure initiator mass spectrometry
AssigneesThe Regents of the University of California, National Technology & Engineering Solutions of Sandia, LLC
InventorsTrent R. Northen, Kai Deng
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Abstract
A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.
Claims23
A method for analysis of enzyme activity in a sample, comprising:
- providing NIMS substrates incorporating one or more of, or simulating H, G, or S lignin units linked by one or more, beta-O-4, 5-5′ (biphenyl), β-5 (phenylcoumaran), β-β, and 4-O-5 (diaryl ether) linkages, and tagging each substrate of the NIMS substrates designed with a unique mass fluorous tag or perfluoronated affinity moiety;
- performing ligninase oxidation reactions on the tagged NIMS substrates designed to generate tagged reaction products using one or more enzymes in the sample;
- and
- identifying and analyzing the tagged reaction products of the ligninase oxidation reactions by nanostructure-initiator mass spectrometry (NIMS).
1 more element
Dependencies are inferred from the claim text.