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US 10,822,630 B2Granted
Process for manipulating the level of glycan content of a glycoprotein
AssigneeAmgen Inc.
InventorsDaniel R. Leiske, Michael T. Trentalange
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Abstract
The present invention provides a method for manipulating the fucosylated glycan content on a recombinant protein.
Claims24
A method for manipulating the fucosylated glycan content on a recombinant protein comprising
- inoculating a bioreactor with mammalian host cells expressing the recombinant protein,
- culturing the host cells in a serum free, chemically defined cell culture medium;
- wherein the cell culture medium includes from 10 to 100 ppb copper and from 50 to 1000 nM manganese,
- harvesting the recombinant protein produced by the host cell.
1 more element
Dependencies are inferred from the claim text.