Polyphosphate-dependent glucokinase and method for preparing glucose 6-phosphate by using same
AssigneeCj Cheiljedang Corporation
InventorsSung Jae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim, Seung Won Park
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Abstract
The present invention relates to a novel high-temperature active thermoresistant polyphosphate-dependent glucokinase with high thermal stability, a composition including the enzyme, and methods for producing glucose 6-phosphate using the enzyme.
Claims10
A method for producing glucose 6-phosphate, comprising (a) contacting a composition comprising a thermoresistant polyphosphate-dependent glucokinase having the amino acid sequence of SEQ ID NO. 2 or having the amino acid sequence which has at least 95% sequence identity to SEQ ID NO. 2 with glucose and polyphosphate at a temperature of 60-80° C.,
- wherein the glucokinase has a maximum activity at 65-70° C. and has activities of 95% or more relative to the maximum activity in the whole range of 60−80° C.
Dependencies are inferred from the claim text.