Simultaneous gene silencing and suppressing gene silencing in the same cell

A plant cell comprising,

• i) a polynucleotide of interest which encodes a target RNA,
• ii) a first exogenous polynucleotide encoding a double stranded RNA (dsRNA) molecule which comprises a stem-loop and a first nucleotide sequence which is complementary to a region of the target RNA encoded by the polynucleotide of interest,
• iii) a second exogenous polynucleotide encoding a silencing suppressor polypeptide,
• iv) a third exogenous polynucleotide, different to the first and second exogenous polynucleotides and the polynucleotide of interest, which encodes an RNA of interest,
• v) a reduced level of the target RNA encoded by the polynucleotide of interest and/or the amount of a protein encoded by the target RNA when compared to a corresponding cell lacking the first exogenous polynucleotide, and
• vi) an increased level of the RNA of interest and/or the amount of protein encoded by the RNA of interest when compared to a corresponding cell having the third exogenous polynucleotide and lacking the second exogenous polynucleotide,
• wherein each exogenous polynucleotide is operably linked to one or more promoters that are capable of directing expression of the polynucleotide in the cell, wherein the first and second exogenous polynucleotides form part of one DNA construct, and wherein the silencing suppressor comprises amino acids having the sequence set forth in SEQ ID NO:
• 1, or a sequence which is at least 95% identical to the sequence set forth in SEQ ID NO:
• 1.