Virus purification
AssigneeKentucky BioProcessing, Inc.
InventorsLeigh Burden, Steven D. Hume, Joshua Morton, Greg Pogue, Barry Bratcher, Hugh A. Haydon, Carrie A. Simpson, Nick Partain, John W. Shepherd
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Abstract
Disclosed herein are methods and exemplary compositions associated with virus purification, exemplary aspects of which may include harvesting viral and antigenic substances from source organisms; and a purification platform comprising chemical separation and size-difference separation for the removal of contaminants, debris and impurities from the viral and protein (e.g. antigenic, including influenza hemagglutinin antigens) substances, as well as their concentration and collection.
Claims10
A method for purifying at least one virus harvested from a source organism, comprising:
- removing cellular debris from the virus by passing the at least one virus through a tangential flow filtration apparatus comprising a porous ceramic filter;
- concentrating the virus;
- and
- performing separations comprising sub-steps of separating host cell contaminants from the virus, subjecting the virus to affinity chromatography to elute the virus, and subjecting the virus to multi-modal chromatography to separate residual impurities from the virus based on size differences between the impurities as retentate and the virus as permeate.
Dependencies are inferred from the claim text.