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US 10,821,446 B1Granted
Fluorescence detector for microfluidic diagnostic system
AssigneeHandyLab, Inc.
InventorsKalyan Handique, Sundaresh N. Brahmasandra
Life of this patent
Estimating the term…
Abstract
The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
Claims20
A system comprising:
- a receiving bay configured to receive a multi-lane microfluidic cartridge;
- a plurality of heaters aligned along a heater axis and configured to apply heat to the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is received in the receiving bay, the plurality of heaters comprising at least a first heater and a second heater;
- the multi-lane microfluidic cartridge disposed over the plurality of heaters when the multi-lane microfluidic cartridge is removably received in the receiving bay, the multi-lane microfluidic cartridge comprising a first portion comprising a plurality of detection regions within which amplification of polynucleotides is carried out, the plurality of detection regions comprising at least a first detection region and a second detection region, the plurality of detection regions aligned along a first cartridge axis, wherein the heater axis and the first cartridge axis are substantially parallel when the multi-lane microfluidic cartridge is removably received in the receiving bay,
- the multi-lane microfluidic cartridge comprising a second portion comprising a plurality of inlets comprising at least a first inlet and a second inlet, the plurality of inlets aligned along a second cartridge axis substantially parallel to the first cartridge axis, the first inlet configured to be loaded with a first sample, the second inlet configured to be loaded with a second sample that is different than the first sample
- wherein the first heater is configured to apply heat to the first detection region in order to amplify one or more polynucleotides when the multi-lane microfluidic cartridge is received in the receiving bay and the first sample is received in the multi-lane microfluidic cartridge, and wherein the second heater is configured to apply heat to the second detection region in order to amplify one or more polynucleotides when the multi-lane microfluidic cartridge is received in the receiving bay and the second sample is received in the multi-lane microfluidic cartridge,
- a liquid dispenser;
- a read head disposed over the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is removably received in the receiving bay, the read head configured to detect the presence of the one or more amplified polynucleotides in the plurality of detection regions of the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is removably received in the receiving bay,
- the read head configured to transmit light onto the first detection region and receive light emitted from the first detection region to detect the presence of one or more amplified polynucleotides in the first detection region of the multi-lane microfluidic cartridge,
- the read head further configured to transmit light onto the second detection region and receive light emitted from the second detection region to detect the presence of one or more amplified polynucleotides in the second detection region of the multi-lane microfluidic cartridge,
- wherein the read head disposed over the multi-lane microfluidic cartridge does not cover the second cartridge axis when the multi-lane microfluidic cartridge is removably received in the receiving bay.
7 more elements
A system comprising:
- a receiving bay configured to receive a multi-lane microfluidic cartridge;
- a plurality of heaters aligned along a heater axis and configured to apply heat to the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is received in the receiving bay, the plurality of heaters comprising at least a first heater and a second heater;
- the multi-lane microfluidic cartridge positioned over the plurality of heaters when the multi-lane microfluidic cartridge is removably received in the receiving bay, the multi-lane microfluidic cartridge comprising a first portion comprising a plurality of detection regions within which amplification of polynucleotides is carried out, the plurality of detection regions comprising at least a first detection region and a second detection region aligned along a first cartridge axis, wherein the heater axis and the first cartridge axis substantially parallel when the multi-lane microfluidic cartridge is removably received in the receiving bay,
- the multi-lane microfluidic cartridge comprising a second portion comprising a plurality of inlets comprising at least a first inlet and a second inlet aligned along a second cartridge axis substantially parallel to the first cartridge axis;
- wherein the first heater is configured to apply heat to the first detection region in order to amplify one or more polynucleotides when the multi-lane microfluidic cartridge is received in the receiving bay and a first sample is received in the multi-lane microfluidic cartridge, and wherein the second heater is configured to apply heat to the second detection region in order to amplify one or more polynucleotides when the multi-lane microfluidic cartridge is received in the receiving bay and a second sample is received in the multi-lane microfluidic cartridge, the second sample different than the first sample,
- a liquid dispenser configured to load the first sample into the first inlet and load the second sample into the second inlet when the multi-lane microfluidic cartridge is removably received in the receiving bay;
- and
- a read head configured to transmit light onto the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is removably received in the receiving bay, the read head configured to transmit light onto the first detection region or onto the second detection region or onto both the first and the second detection regions while the read head is positioned over the first portion of the multi-lane microfluidic cartridge,
- wherein, when the multi-lane microfluidic cartridge is removably received in the receiving bay, the read head is further configured to receive light emitted from the first detection region or light emitted from the second detection region or light emitted from both the first and the second detection regions to detect the presence of the one or more amplified polynucleotides in the first detection region of the multi-lane microfluidic cartridge or in the second detection region of the multi-lane microfluidic cartridge or in both the first and the second detection regions of the multi-lane microfluidic cartridge while the read head is positioned over the first portion of the multi-lane microfluidic cartridge, and
- wherein the read head positioned over the first portion of the multi-lane microfluidic cartridge does not cover the second cartridge axis when the multi-lane microfluidic cartridge is removably received in the receiving bay.
7 more elements
Dependencies are inferred from the claim text.