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US 10,821,134 B2Granted
BK virus specific T cells
AssigneeBoard of Regents, The University of Texas System
InventorsKaty Rezvani, Elizabeth Shpall, Muharrem Muftuoglu
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Abstract
Provided herein are methods of rapidly expanding BKV-specific T cells using a peptide mixture and cytokines. Further provided herein are methods of treating polyomavirus-associated diseases by administering the BKV-specific T cells.
Claims21
An ex vivo method for rapid expansion of BK virus (BKV)-specific T cells comprising the step of:
- directly stimulating in culture a population of isolated peripheral blood mononuclear cells (PBMCs) in the presence of a mixture of peptides, IL-7, IL-15, and IL-2, wherein the mixture of peptides comprises overlapping peptides spanning at least 3 immunodominant BKV proteins selected from the group consisting of small T antigen, large T antigen, VP1, VP2, and VP3, thereby producing a cultured cell population comprising expanded BKV-specific T cells wherein the culture does not comprise isolated antigen presenting cells (APCs) pre-stimulated with the mixture of peptides, and wherein the peptides, IL-7, IL-15, and IL-2 are added to the culture substantially simultaneously.
Dependencies are inferred from the claim text.