US 10,816,468 B2 · Flash photo-oxidation device and higher order structural analysis

Abstract

Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.

Claims21

Dependent claims

A sample analysis system comprising:

a flash photolysis system configured for irradiating a sample containing a photo-reactive reagent mixture;
a photolysis cell in optical communication with the flash photolysis system and being configured to receive the sample and for the sample to be irradiated by the flash photolysis system while the sample is in the photolysis cell to cause a photolytic reaction of the sample;
a radical dosimeter configured for irradiating products of the photolytic reaction and detecting a photometric property of the products;
a product vessel configured to receive the products of the photolytic reaction, the photolysis cell, radical dosimeter and product vessel being in-line;
and
a feed-back closed-loop control circuit configured to control a spectral irradiance of the flash photolysis system in real-time in accordance with a change in the photometric property of the products as measured by the radical dosimeter.

3 more elements

Dependencies are inferred from the claim text.

Flash photo-oxidation device and higher order structural analysis

Life of this patent

Flash photo-oxidation device and higher order structural analysis

Life of this patent

Abstract

Claims21