Microorganism of the genus Escherichia producing L-tryptophan and method for producing L-tryptophan using the same
AssigneeCj Cheiljedang Corporation
InventorsKi Yong Cheong, Kyung Rim Kim, Seok Myung Lee, Kwang Ho Lee, Keun Cheol Lee, Hyeong Pyo Hong
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Abstract
The present application relates to a microorganism of the genus Escherichia producing L-tryptophan and, more specifically, to a microorganism of the genus Escherichia with improved activity of producing L-tryptophan by weakening or inactivating the activity of endogenous 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Additionally, the present application relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.
Claims10
A microorganism of the genus Escherichia producing L-tryptophan, wherein the activities of endogenous 6-phosphogluconate dehydratase (Edd) and 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) are weakened or inactivated, wherein the microorganism has a weakened or blocked Entner-Doudoroff pathway thereby does not lose 6-phosphogluconate, and wherein an entirety or a part of the pheA gene, trpR gene, mtr gene, and tnaAB gene is further deleted.
A method of preparing L-tryptophan, comprising:
- culturing a microorganism of the genus Escherichia producing L-tryptophan, wherein the activities of endogenous 6-phosphogluconate dehydratase (Edd) and 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) are weakened or inactivated, wherein the microorganism has a blocked Entner-Doudoroff pathway thereby does not lose 6-phosphogluconate in a medium, and wherein the microorganism has higher L-tryptophan production relative to an identical microorganism with the proviso the Edd and Eda are not weakened or inactivated;
- and recovering L-tryptophan from the cultured medium or the cultured microorganism.
Dependencies are inferred from the claim text.