Method for obtaining active insoluble xylose isomerase
AssigneeMetgen Oy
InventorsKlara Birikh, Anu Minna Maaret Suonpää
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Abstract
The invention is in the field of biotechnology and involves recombinant DNA technology. It provides means and methods for obtaining an insoluble active fusion protein comprising xylose isomerase activity. More in particular, the invention relates to a method for obtaining active insoluble xylose isomerase, comprising the expression in a host organism of a recombinant gene encoding a fusion protein comprising a xylose isomerase in combination with a PPIase, thereby obtaining the active insoluble xylose isomerase. It also provides recombinant fusion proteins comprising xylose isomerase activity as well as their use in converting xylose to xylulose and glucose to fructose.
Claims11
A method for obtaining an active insoluble xylose isomerase, the method comprising:
- expressing, in E. coli, a recombinant gene encoding a fusion protein;
- wherein the fusion protein comprises a xylose isomerase linked to SEQ ID NO:
- 22,
- thereby obtaining the active insoluble xylose isomerase.
Dependencies are inferred from the claim text.