Modified helicases
AssigneeOxford Nanopore Technologies PLC
InventorsAndrew Heron, James Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, David Alves, Rebecca Bowen, John Milton
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Abstract
The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Claims12
A complex comprising (i) a monomeric helicase that comprises a polynucleotide binding domain and two RecA-like domains, and (ii) a target polynucleotide bound to the polynucleotide binding domain, wherein two amino acid residues that are located in different structural domains on the surface of the helicase surrounding the polynucleotide binding domain are artificially covalently connected via a linkage between the two amino acid residues, such that the helicase has a covalently-closed structure and the distance between the two amino acids is less than 50 Angstroms (Å), wherein the bound target polynucleotide is encircled by the covalently-closed structure.
Dependencies are inferred from the claim text.