Selection methods for genetically-modified T cells
AssigneeBoard of Regents, The University of Texas System
InventorsDavid Rushworth, Laurence J. N. Cooper
Life of this patent
Estimating the term…
Abstract
In some aspects, isolated transgenic cells (e.g., transgenic T cells) are provided that comprise or express a transgene and DHFRFS and/or TYMSSS. Methods for selecting transgenic cells are also provided.
Claims19
An isolated engineered mammalian T cell comprising a protein comprising the amino acid sequence of SEQ ID NO:12 (dihydrofolate reductaseFS;
- DHFRFS) and a protein comprising the amino acid sequence of SEQ ID NO:
- 11 (thymidylate synthaseSS;
- TYMSSS).
An isolated engineered mammalian T cell comprising a nucleotide sequence comprising a transgene of interest and a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 12 (dihydrofolate reductaseFS;
- DHFRFS) and a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 11 (thymidylate synthaseSS;
- TYMSSS).
A method for providing controlled expression of a first transgene comprising providing an engineered mammalian cell comprising a nucleic acid comprising the first transgene operably linked to a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 11 (thymidylate synthaseSS;
- TYMSSS), said cell further comprising a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 12 (dihydrofolate reductaseFS;
- DHFRFS).
Dependencies are inferred from the claim text.