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US 10,806,150 B2Granted
Methods and compositions of insect control
AssigneeApse, Inc.
InventorsJohn L. Killmer, Juan Pedro Humberto Arhancet, Patrick D. McLaughlin
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AssigneeApse, Inc.
InventorsJohn L. Killmer, Juan Pedro Humberto Arhancet, Patrick D. McLaughlin
Estimating the term…
The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like Particles (VLPs) suitable for insect control applications. Specifically, the disclosure provides a method for controlling target insects comprising, transforming a microbial host with a first DNA sequence comprising a gene encoding a bacteriophage capsid protein and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the microbial host to express the first and second DNA sequences, isolating virus-like-particles (VLPs) comprising the capsid protein and RNAi precursor from the microbial host, and contacting the isolated VLPs with the target insects.
A method for controlling target insects comprising, transforming a bacterium with a first DNA sequence comprising a gene encoding a levivirus Qβ capsid protein or a levivirus MS2 capsid protein and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the bacterium to express the first and second DNA sequences, isolating virus-like-particles (VLPs) consisting of the capsid protein enclosing the RNAi precursor from the bacterium, and contacting the isolated VLPs with the target insects, wherein the target insect comprises a Coleopteran insect or a Lepidopteran insect.
Dependencies are inferred from the claim text.