Microorganism modified for the assimilation of levulinic acid
AssigneeMetabolic Explorer
InventorsGwenaëlle Corre, Laurence Dumon-Seignovert, Philippe Soucaille
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Abstract
The present invention relates to a genetically modified microorganism for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, and to a fermentation process for performing said conversion.
Claims13
A method for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, comprising the step of culturing, under fermentation conditions, a genetically modified microorganism, in a culture medium comprising as a source of carbon at least levulinic acid,
- wherein said genetically modified microorganism overexpresses relative to a corresponding non-genetically modified microorganism:
- at least one enzyme converting levulinic acid into levulinyl-CoA selected from acyl CoA:
- 3-ketoacid CoA/acetate CoA transferases (EC 2.8.3.5/EC 2.8.3.8) and combinations thereof, wherein said at least one enzyme comprises the amino acid sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 5, and
- at least one enzyme converting said levulinyl-CoA into propionyl-CoA and acetyl-CoA, selected from acetyl-CoA C-acetyltransferases (EC 2.3.1.9/EC 2.3.1.16) and combinations thereof, wherein said at least one enzyme comprises the amino acid sequence of SEQ ID NO:
- 9.
A method for the fermentative production of 1,2-propanediol, said method comprising the steps of:
- a) culturing, under fermentation conditions, a genetically modified microorganism, in a culture medium comprising as a source of carbon at least levulinic acid, and
- b) recovering said 1,2-propanediol,
- wherein said genetically modified microorganism overexpresses relative to a corresponding non-genetically modified microorganism:
- at least one enzyme converting levulinic acid into levulinyl-CoA selected from acyl CoA:
- 3-ketoacid CoA/acetate CoA transferases (EC 2.8.3.5/EC 2.8.3.8) and combinations thereof, wherein said at least one enzyme comprises the amino acid sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 5, and
- at least one enzyme converting said levulinyl-CoA into propionyl-CoA and acetyl-CoA, selected from acetyl-CoA C-acetyltransferases (EC 2.3.1.9/EC 2.3.1.16) and combinations thereof, wherein said at least one enzyme comprises the amino acid sequence of SEQ ID NO:
- 9.
Dependencies are inferred from the claim text.