Genetically engineered microorganisms and processes for the production of cannabinoids from a carbon source precursor
AssigneeSyntiva Therapeutics, Inc.
InventorsAdam Davidovich, Brian Chan, Neil Gupta, Alim Ladha
Life of this patent
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Abstract
A method is provided for biosynthetic production of cannabinoids in microorganisms from a carbon source precursor. This method describes the genetic modifications needed to engineer microorganisms to produce cannabinoids as well as a method for identifying and quantifying cannabinoids from fermentation broth. A system is also provided for tuning the method to produce different cannabinoids of interest by systematically modulating the enzymes encoded by the genetic modifications introduced in the microorganism.
Claims13
A genetically modified microorganism that produces at least one cannabinoid from a carbon source precursor, wherein the microorganism is E. coli comprising a recombinantly incorporated mutated FadD gene having a nucleotide sequence of SEQ ID NO:
- 10 at the genomic location of a FadE gene of the microorganism to express the mutated FadD enzyme and simultaneously knock out the FadE gene of the microorganism.
A plasmid that encodes olivetol synthase having an amino acid sequence of SEQ ID NO:
- 2, olivetolic acid cyclase having an amino acid sequence of SEQ ID NO:
- 4, CsPT1 having an amino acid sequence of SEQ ID NO:
- 6, and a Glutathione-S-Transferase solubility tag having an amino acid sequence of SEQ ID NO:
- 8.
A genetically modified microorganism that produces at least one cannabinoid from a carbon source precursor, wherein the microorganism is S. cerevisiae and has been transformed with a plasmid to express a polypeptide encoding olivetol synthase having the amino acid sequence of SEQ ID NO:
- 2, a polypeptide encoding olivetolic acid cyclase having the amino acid sequence of SEQ ID NO:
- 4, a polypeptide encoding CsPT1 having the amino acid sequence of SEQ ID NO:
- 6, and further comprising a Glutathione-S-Transferase (GST) solubility tag with a thrombin cleavage site having the amino acid sequence of SEQ ID NO:
- 8 in the same open reading frame as CsPT1.
Dependencies are inferred from the claim text.