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US 10,801,048 B2Granted
Method of producing L-amino acids
AssigneeCj Cheiljedang Corporation
InventorsJun Ok Moon, Sang Jo Lim, Do Hyun Kwon, Kwang Ho Lee, Hyun Won Bae
Life of this patent
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Abstract
Provided is a method of producing L-amino acids by using a recombinant coryneform microorganism in which the expression of a target gene is weakened by using a gene transcription inhibition method.
Claims7
A method of producing a target L-amino acids, the method comprising
- culturing a recombinant coryneform microorganism in a medium, wherein the recombinant coryneform microorganism is capable of producing a target L-amino acid and is transformed by inserting an acetate-inducible promoter downstream of a stop codon of a target gene in a chromosome such that transcription from the acetate-inducible promoter occurs in a direction opposite to the direction of the target gene transcription, wherein the target gene is a gene located at a branch point of a biosynthetic pathway of the target L-amino acid and encoding an enzyme involved in byproduct production, wherein the target L-amino acid is L-threonine, L-methionine, L-isoleucine, L-lysine, L-alanine, or L-valine.
Dependencies are inferred from the claim text.