Methods for making chemoautotrophic cells by engineering an energy conversion pathway and a carbon fixation pathway
AssigneeGinkgo Bioworks, Inc.
InventorsCurt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Abstract
The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Claims15
A method of making a chemoautotrophic cell, the method comprising steps of:
- (i) engineering in a cell an energy conversion pathway comprising a recombinant formate dehydrogenase or a recombinant sulfide-quinone oxidoreductase, wherein the recombinant formate dehydrogenase is encoded by any one of SEQ ID NOs:1-4 or a homolog thereof having at least 80% sequence identity thereto, or comprises any one of SEQ ID NOs:5-8 or a homolog thereof having at least 80% sequence identity thereto, and the recombinant sulfide-quinone oxidoreductase is encoded by any one of SEQ ID NOs:9-16 or a homolog thereof having at least 80% sequence identity thereto;
- wherein the energy conversion pathway transfers energy from an extracellular inorganic energy source to a reducing cofactor, and
- (ii) engineering in the cell a carbon fixation pathway that utilizes the reducing cofactor to convert inorganic carbon to a reduced organic carbon molecule.
Dependencies are inferred from the claim text.