Exporting a selected group of micro-objects from a micro-fluidic device

A process of exporting micro-objects from a micro-fluidic apparatus comprising:

• an enclosure comprising a base and a micro-fluidic structure disposed on said base, wherein said enclosure defines a flow region configured to contain a liquid medium and a plurality of holding pens,
• wherein said flow region comprises a channel, wherein each holding pen of said plurality opens off of said channel and comprises an isolation region and a connection region, with said isolation region fluidically connected to said channel via said connection region, and wherein said isolation region is configured to hold a group of micro-objects and isolate said group of micro-objects from micro-objects in other holding pens of said plurality,
• wherein said enclosure further comprises
• an inlet and an outlet, each associated with said flow region, for respectively inputting and removing said liquid medium from said flow region, said outlet providing a passage through said enclosure;
• and
• a first electrode, a second electrode, and an electrode activation substrate comprising DEP electrodes located at an inner surface of said flow region, said process comprising:
• selecting a group of micro-objects located in one holding pen of said plurality, wherein said selected group of micro-objects comprises a plurality of biological cells from a clonal colony of biological cells;
• moving said selected group of micro-objects into said channel;
• and
• exporting said selected group of micro-objects by flowing said liquid medium in said channel, wherein said export flow of said liquid medium carries said selected group of micro-objects to said outlet and through said passage through said enclosure.