Artificial tissue and method for producing same
AssigneesOsaka University, Tohoku University
InventorsMitsuru Akashi, Michiya Matsusaki, Michiaki Unno, Naoaki Sakata, Gumpei Yoshimatsu
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Abstract
An artificial tissue containing pancreatic islet cells, fibroblasts and/or cells capable of differentiating, extracellular matrix, and vascular endothelial cells, in which the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute a three-dimensional tissue structure in which a vascular network structure has been formed, and in which the three-dimensional tissue structure contains pancreatic islets constituted by aggregating ten or more of the pancreatic islet cells.
Claims6
A method for production of an artificial tissue, comprising:
- culturing fibroblasts and/or the cells capable of differentiating and vascular endothelial cells with a composition comprising fibronectin and gelatin, wherein the composition comprising fibronectin and gelatin coats the fibroblasts and/or the cells capable of differentiating and vascular endothelial cells;
- and
- culturing pancreatic islet cells arranged on a cell layer formed by the fibroblasts and/or the cells capable of differentiating and vascular endothelial cells;
- and
- forming a three-dimensional tissue structure by culturing the fibroblasts and/or cells capable of differentiating and the vascular endothelial cells with extracellular matrix, wherein the extracellular matrix is a combination of fibronectin and gelatin, wherein the extracellular matrix coats the three-dimensional tissue structure;
- and
- culturing pancreatic islet cells arranged on the three-dimensional tissue structure that has been formed, wherein:
- the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute the three-dimensional tissue structure in which a vascular network structure has been formed;
- and
- the three-dimensional tissue structure contains pancreatic islets constituted by aggregating a plurality of the pancreatic islet cells.
A method for production of an artificial tissue, comprising:
- arranging and culturing fibroblasts and/or the cells capable of differentiating with a composition comprising fibronectin and gelatin, wherein the composition comprising fibronectin and gelatin coats the fibroblasts and/or the cells capable of differentiating;
- and
- culturing vascular endothelial cells on a cell layer formed by the fibroblasts and/or the cells capable of differentiating with a composition comprising fibronectin and gelatin, wherein the composition comprising fibronectin and gelatin coats the vascular endothelial cells;
- and
- forming a three-dimensional. tissue structure by culturing the fibroblasts and/or cells capable of differentiating and the vascular endothelial cells with extracellular matrix, wherein the extracellular matrix is a combination of fibronectin and gelatin, wherein the extracellular matrix coats the three-dimensional tissue structure;
- and
- culturing pancreatic islet cells arranged on the three-dimensional tissue structure that has been formed, wherein:
- the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute the three-dimensional tissue structure in which a vascular network structure has been formed;
- and
- the three-dimensional tissue structure contains pancreatic islets constituted by aggregating a plurality of the pancreatic islet cells.
Dependencies are inferred from the claim text.