Method of packing polynucleotides

A method of preparing nanoparticle polynucleotides, the method comprising forming the polynucleotides comprising modified nucleotides, wherein the forming comprises chemically synthesizing the polynucleotides comprising modified nucleotides, synthesizing the polynucleotides comprising modified nucleotides using an enzyme, or a combination thereof, and wherein the polynucleotides comprise at least partially a nucleotide sequence forming a secondary structure, a tertiary structure, a quaternary structure, or a combination thereof as a biomolecular structure, wherein the modified nucleotides have a modification of the hydrogen of the 8-carbon site of the purine ring when a base of the modified nucleotides is adenine or guanine, of the hydrogen of the 5-carbon site of the pyrimidine ring when a base of the modified nucleotides is cytosine or uracil, and of the methyl group of the 5-carbon site of the pyrimidine ring when a base of the modified nucleotides is thymine, the modified nucleotides being modified by substituting the hydrogen or the methyl group with a cationic residue.