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US 10,793,881 B2Granted
Method for the microbial production of 8-methyl nonanoic acid
AssigneeConagen Inc.
InventorsHui Chen, Xiaodan Yu
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Abstract
The present disclosure relates to the production of 8-methyl nonanoic acid and medium chain branched fatty acids, e.g., via microbial fermentation.
Claims16
A biosynthetic method of producing 8-methyl nonanoic acid (8M) comprising:
- a) expressing a KASIIIa gene, a KASIIIb gene and one or more genes encoding an acyl-acyl carrier protein (ACP) thioesterase in a transformed cellular system;
- b) feeding or producing isobutyryl-CoA in said transformed cellular system;
- and,
- c) producing 8-methyl nonanoic acid;
- wherein the KASIIIa gene comprises a nucleic acid sequence that is at least 90% identical to SEQ ID NO. 6 and the KASIIIb gene comprises a nucleic acid sequence that is at least 90% identical to SEQ ID NO. 7.
2 more elements
Dependencies are inferred from the claim text.