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US 10,793,880 B2Granted
Production of frambinone by a recombinant fungal microorganism
AssigneeLesaffre Et Compagnie
InventorsAlexander Matthias Farwick, Thomas Desfougeres, Georges Pignede, Anaïs Roussel, Isabelle Mouly, Brieuc Morvan
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Abstract
The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
Claims16
A genetically modified fungal microorganism for the production of frambinone, said microorganism having the following characteristics:
- a capacity to produce frambinone from tyrosine;
- and
- a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate,
- wherein said genetically modified fungal microorganism has hydroxyphenyl pyruvate decarboxylase (HPPDC) activity less than or equal to 2×10−6 kat per g of protein, and comprises at least one mutation or deletion in at least one of the genes encoding the following enzymes:
- deaminase Aro8, deaminase Aro9, decarboxylase Aro10, decarboxylase Pdc5, decarboxylase Pdc6, or alcohol dehydrogenase (ADH).
2 more elements
Dependencies are inferred from the claim text.