US 10,793,872 B2 · HPPD variants and methods of use

Abstract

Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

Claims22

Dependent claims

A recombinant nucleic acid molecule encoding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, wherein said HPPD polypeptide has an amino acid sequence having at least 95% sequence identity to SEQ ID NO:1, wherein said amino acid sequence comprises the following amino acid substitutions

a proline at the amino acid position corresponding to amino acid position 335 of SEQ ID NO:1, a tryptophan at the amino acid position corresponding to amino acid position 336 of SEQ ID NO:
1, an alanine at the amino acid position corresponding to amino acid position 339 of SEQ ID NO:1, and a glutamine at the amino acid position corresponding to amino acid position 340 of SEQ ID NO:1;
wherein said HPPD polypeptide, when expressed in a soybean plant, confers to said plant tolerance to 200 g AI/ha tembotrione.

Dependencies are inferred from the claim text.

Facts

Application

14/441,638Filed 13 Sep 2013 · us provisional application

Priority

14 Sep 2012

Published

US 2015/0267180 A124 Sep 2015

Granted

6 Oct 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/8274
For stress resistance, e.g. heavy metal resistance: for herbicide resistance
C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C12N 15/8209
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12Y 113/11027
With incorporation of two atoms of oxygen: 4-Hydroxyphenylpyruvate dioxygenase

Family

Application61/701,037Us provisional application · filed 14 Sep 2012

Application61/766,057Us provisional application · filed 18 Feb 2013

Application61/790,404Us provisional application · filed 15 Mar 2013

US 2015/0267180 A1Pre-grant publication · published 24 Sep 2015

US 10,793,872 B2This patent

From the US filing history. Foreign family members aren’t listed.

HPPD variants and methods of use

Life of this patent

HPPD variants and methods of use

Life of this patent

Abstract

Claims22