Synthesis and oxidation of methane
AssigneeAuburn University
InventorSteven Mansoorabadi
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Abstract
The present disclosure describes genes and proteins of the coenzyme F430 synthetic pathway. The genes and proteins in the pathway find uses as isolated nucleic acids, transformation vectors, a transformation media, genetically modified cells, methods of modulating methanogenesis, methods of modulating methane oxidation, methods of making a tetrapyrrole compound, methods of oxidizing methane, methods of biogenic methane synthesis is provided, methods of assaying an organism for potential methanogenic or methanotrophic activity, and isolated proteins.
Claims19
A cell comprising a heterologous polynucleotide encoding a polypeptide having at least 70% sequence identity with a first Cfb polypeptide selected from Table 1;
- and comprising a second polynucleotide encoding a second polypeptide having at least 70% sequence identity with a second Cfb polypeptide selected from Table 1 wherein the second Cfb polypeptide is not the same as the first Cfb polypeptide.
A cell comprising a polynucleotide encoding a polypeptide having at least 70% sequence identity with a first Cfb polypeptide selected from Table 1, wherein said polypeptide is operatively linked to a heterologous promoter;
- and comprising a second polynucleotide encoding a second polypeptide having at least 70% sequence identity with a second Cfb polypeptide selected from Table 1;
- wherein the second Cfb polypeptide is not the same as the first Cfb polypeptide.
A cell comprising:
- a polynucleotide encoding a first polypeptide having at least 70% sequence identity with a CfbA polypeptide selected from Table 1;
- a second polynucleotide encoding a second polypeptide having at least 70% sequence identity with a CfbB polypeptide selected from Table 1;
- a third polynucleotide encoding a third polypeptide having at least 70% sequence identity with a CfbC polypeptide selected from Table 1;
- a fourth polynucleotide encoding a fourth polypeptide having at least 70% sequence identity with a CfbD polypeptide selected from Table 1;
- a fifth polynucleotide encoding a fifth polypeptide having at least 70% sequence identity with a CfbE polypeptide selected from Table 1;
- and a sixth polypeptide encoding a sixth polypeptide having at least 70% sequence identity with a sixth Cfb polypeptide selected from Table 1.
Dependencies are inferred from the claim text.