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US 10,792,395 B2Granted
Methods and devices for the production of decellularised tissue scaffolds
AssigneeUCL Business PLC
InventorsGiuseppe Mazza, Walid Al-Akkad Abu Zeina, Massimo Pinzani
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Abstract
This invention relates to a method of producing a decellularised tissue scaffold which comprising treating a sample of tissue with an osmotic reagent and a detergent whilst subjecting the tissue sample to oscillation with a displacement of 1 mm or more and a frequency of 3 Hz or more during these treatment steps. This method may be useful in producing acellular scaffolds that maintain the 3-D architecture and extracellular matrix composition and morphology of the native tissue.
Claims32
A method of producing a decellularised tissue scaffold comprising;
- (i) providing a tissue sample selected from the group consisting of kidney, muscle, bone, adipose, cartilage, lung, bladder, cornea, skin, liver, spleen, placenta, intestine, pancreas, prostate, breast and heart,
- (ii) treating the tissue sample with an osmotic reagent, and
- (iii) treating the tissue sample obtained from step (ii) with a detergent,
- wherein steps (ii) and (iii) are repeated, and wherein the tissue sample is subjected to oscillation in a single linear dimension with a displacement of 1 mm or more and a frequency of 3 to 100 Hz during steps (ii) and (iii), said oscillation subjecting the tissue sample to a g-force of 6 to 50 ms−2 thereby producing a decellularised tissue scaffold.
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