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US 10,792,378 B2Granted
Using a blue-shifted reference dye in an optical glucose assay
AssigneeMedtronics MiniMed, Inc.
InventorsSoren Aasmul, Jesper Svenning Kristensen
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Abstract
The invention is directed to a competitive glucose binding affinity assay comprising a glucose receptor (typically mannan binding lectin) labeled with an assay fluorophore and a modified glucose analog (typically dextran) labeled with a reference fluorophore. In certain embodiments, the glucose analog is dextran and is coupled to both a reference fluorophore and a quencher dye (e.g. hexamethoxy crystalviolet-1). Optionally the reference fluorophore is blue shifted relative to the assay fluorophore.
Claims8
A glucose sensing complex comprising:
- a glucose binding agent coupled to an assay fluorophore;
- and
- a glucose analog coupled to a reference fluorophore;
- wherein:
- the reference fluorophore is blue shifted relative to the assay fluorophore;
- the assay fluorophore exhibits a wavelength that is at least 50 nanometers greater than the wavelength of the reference fluorophore;
- and
- the assay fluorophore and reference fluorophore are individually selected from the group consisting of Alexa Fluor 594 (AF594), Alexa Fluor 647 (AF647), and Alexa Fluor 700 (AF700), and wherein the reference fluorophore has a lower wavelength than the assay fluorophore.
5 more elements
Dependencies are inferred from the claim text.