Electrochemical test device
AssigneeInside Biometrics International Limited
InventorsMarco Cardosi, Stephanie Kirkwood, Damian Baskeyfield
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Abstract
An electrochemical test device for use in determining a concentration of each of a first analyte and a second analyte in a fluid sample is provided. The electrochemical test device comprises a set of electrodes including a first working electrode having sensing chemistry for the first analyte and a second working electrode having sensing chemistry for the second analyte, wherein the first analyte is lactate and the sensing chemistry for the lactate comprises lactate oxidase and an electron transfer agent, and wherein the sensing chemistry for the second analyte comprises a diaphorase, an electron transfer agent, an NAD(P)+-dependent dehydrogenase and a cofactor for the NAD(P)+-dependent dehydrogenase.
Claims20
An electrochemical test device for use in determining a concentration of each of a first analyte and a second analyte in a fluid sample, the electrochemical test device comprising a set of electrodes including a first working electrode having sensing chemistry for the first analyte, a second working electrode having sensing chemistry for the second analyte, and a counter/reference electrode configured to establish a current between each of the first and second working electrode, wherein:
- the first analyte is lactate and the sensing chemistry for the lactate comprises lactate oxidase and an electron transfer agent;
- the sensing chemistry for the second analyte comprises a diaphorase, a second electron transfer agent having a second redox potential, an NAD(P)+-dependent dehydrogenase and a cofactor for the NAD(P)+-dependent dehydrogenase;
- and
- the counter/reference electrode is provided with a third electron transfer agent having a third redox potential, wherein the third standard redox potential is higher than the second standard redox potential by at least 0.2V and wherein the first standard redox potential is substantially the same as the third standard redox potential.
A method of determining a concentration of a first analyte in a fluid sample and a concentration of a second analyte in the fluid sample, wherein an electrochemical test device is used, the device comprising a set of electrodes comprising a first working electrode, a second working electrode and a counter/reference electrode configured to establish a current between each of the first and second working electrode, wherein the first working electrode is provided with first sensing chemistry for the first analyte, the first sensing chemistry including a first electron transfer agent, and wherein the second working electrode is provided with second sensing chemistry for the second analyte, the second sensing chemistry including a second electron transfer agent, wherein the counter/reference electrode is provided with a third electron transfer agent, wherein the first electron transfer agent has a first standard redox potential, the second electron transfer agent has a second standard redox potential and the third electron transfer agent has a third standard redox potential, wherein the third standard redox potential is higher than the second standard redox potential by at least 0.2V, wherein the first standard redox potential is substantially the same as the third standard redox potential, the method comprising:
- operating in a fuel cell mode for a first time period in which the difference between the second standard redox potential and the third standard redox potential causes current to flow from the second working electrode to the counter/reference electrode, wherein the first time period serves as a poise delay period for the first working electrode;
- after the first time period, applying a potential difference for a second time period between the first working electrode and the counter/reference electrode;
- determining the concentration of the second analyte based on an output signal generated from the current that flows between the second working electrode and the counter/reference electrode in the first time period;
- and
- determining the concentration of the first analyte based on an output signal generated from the current that flows between the first working electrode and the counter/reference electrode in the second time period.
An electrochemical test device for use in determining a concentration of a first analyte in a fluid sample and a concentration of a second analyte in the fluid sample, the device comprising a set of electrodes comprising a first working electrode, a second working electrode and a counter/reference electrode configured to establish a current between each of the first and second working electrode, wherein the first working electrode is provided with first sensing chemistry for the first analyte, the first sensing chemistry including a first electron transfer agent, and wherein the second working electrode is provided with second sensing chemistry for the second analyte, the second sensing chemistry including a second electron transfer agent, wherein the counter/reference electrode is provided with a third electron transfer agent, wherein the first electron transfer agent has a first standard redox potential, the second electron transfer agent has a second standard redox potential and the third electron transfer agent has a third standard redox potential, wherein the third standard redox potential is higher than the second standard redox potential by at least 0.2V, and wherein the first standard redox potential is substantially the same as the third standard redox potential.
Dependencies are inferred from the claim text.