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US 10,787,691 B2Granted
Method for producing L-amino acid
AssigneeAjinomoto Co., Inc.
InventorsHiromi Noguchi, Yo Nishiyama, Yoshihiro Ito
Life of this patent
Estimating the term…
Abstract
A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing in a medium a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a c1795 protein is reduced or the activity of a protein of which the expression is repressed by a c1795 protein is increased, and collecting the L-amino acid from the medium or cells of the bacterium.
Claims22
A bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability,
- wherein the bacterium has a feature selected from the group consisting of:
- (A) the bacterium has been modified so that the activity of a c1795 protein is reduced;
- and
- (B) the bacterium has been modified so that the activity of a protein P is increased, wherein the expression of protein P is repressed by a c1795 protein;
- wherein the c1795 protein and the protein P are native to a bacterium belonging to the genus Pantoea.
2 more elements
A bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability,
- wherein the bacterium has a feature selected from the group consisting of:
- (A) the bacterium has been modified so that the activity of a c1795 protein is reduced;
- and
- (B) the bacterium has been modified so that the activity of a protein P is increased, wherein the expression of protein P is repressed by a c1795 protein,
- wherein the protein P is a protein selected from the group consisting of a PAJ_1175 protein, a PAJ_1174 protein, a PAJ_1173 protein, and combinations thereof,
- wherein the c1795 protein is selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 2, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having a function of a c1795 protein;
- and
- (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 2, and having a function of a c1795 protein;
- wherein the function of a c1795 protein is as a transcriptional regulator of the Rrf2 family,
- wherein the PAJ_1175 protein is selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 4;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 4, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having a function of a PAJ_1175 protein;
- and
- (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 4, and having a function of a PAJ_1175 protein;
- wherein the function of a PAJ_1175 protein is as a transcriptional regulator of the AraC family,
- wherein the PAJ_1174 protein is selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 6;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 6, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having a function of a PAJ_1174 protein;
- and
- (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 6, and having a function of a PAJ_1174 protein;
- wherein the function of a PAJ_1174 protein is as a periplasm adapter subunit of a multi-drug efflux transporter belonging to the RND (resistance-nodulation-cell division) superfamily,
- wherein the PAJ_1173 protein is selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 8;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 8, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having a function of a PAJ_1173 protein;
- and
- (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 8, and having a function of a PAJ_1173 protein;
- wherein the function of a PAJ_1173 protein is as a permease subunit of a multi-drug efflux transporter belonging to the RND (resistance-nodulation-cell division) superfamily.
38 more elements
Dependencies are inferred from the claim text.