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US 10,787,690 B2Granted
Microorganism of the genus Corynebacterium producing l-lysine and a method for producing l-lysine using the same
AssigneeCj Cheiljedang Corporation
InventorsHyo Jeong Byun, Hyung Joon Kim, Hyun Won Bae, Song-Gi Ryu, Hyang Choi, Jun Ok Moon, Kyung-Chang Lee, Yunjung Choi
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Abstract
The present disclosure relates to a microorganism producing L-lysine, and a method for producing L-lysine using the same.
Claims4
An L-lysine producing microorganism of the genus Corynebacterium, having an improved activity of a protein consisting of the amino acid sequence of SEQ ID NO:
- 1 compared to its endogenous activity, wherein the improved activity is selected from the group consisting of:
- (1) an increased copy number of a polynucleotide encoding SEQ ID NO:
- 1;
- (2) increased expression of SEQ ID NO:
- 1 by modification of an expression regulatory sequence, (3) on a chromosome, modification of the expression regulatory sequence or introduction of an alternative expression regulatory sequence so that protein activity of SEQ ID NO:
- 1 is enhanced compared to prior to the modification, (4) codon optimization of the polynucleotide encoding SEQ ID NO:
- 1, and (5) combinations thereof;
- and wherein the improved activity of said SEQ ID NO:
- 1 results in increased L-lysine production in said Corynebacterium compared to an L-lysine producing Corynebacterium that is not modified to have said improved activity.
6 more elements
A method for producing L-lysine, comprising:
- culturing an L-lysine producing microorganism of the genus Corynebacterium, having an improved activity of a protein consisting of the amino acid sequence of SEQ ID NO:
- 1 compared to its endogenous activity, in a medium;
- and recovering L-lysine from the cultured microorganism or medium thereof, wherein the improved activity is selected from the group consisting of:
- (1) an increased copy number of a polynucleotide encoding SEQ ID NO:
- 1;
- (2) increased expression of SEQ ID NO:
- 1 by modification of an expression regulatory sequence, (3) on a chromosome, modification of the expression regulatory sequence or introduction of an alternative expression regulatory sequence so that protein activity of SEQ ID NO:
- 1 is enhanced compared to prior to the modification, (4) codon optimization of the polynucleotide encoding SEQ ID NO:
- 1, and (5) combinations thereof;
- and wherein the improved activity of said SEQ ID NO:
- 1 results in increased L-lysine production in said Corynebacterium compared to an L-lysine producing Corynebacterium that is not modified to have said improved activity.
8 more elements
Dependencies are inferred from the claim text.