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US 10,787,579 B2Granted
Sensors and separation based on molecular recognition via electropolymerization and colloidal layer templates
AssigneeUniversity of Houston System
InventorsRigoberto Advincula, Roderick B. Pernites
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Abstract
Novel coatings are disclosed prepared from electropolymerization of electropolymerizable monomers-analyte complexes onto a conducting layer or non-conducting of a substrate, where the analyte is removed by electrochemically mediated washing permitting linear molecular sensing of the analyte over a wide concentration range. The coating may also include templating particles deposited on the electrode substrate prior to electropolymerization, where the particles can be removed to form a submicron structured coating. Methods for making and using the coatings are also disclosed.
Claims16
A method comprising:
- forming a conducting layer on a surface of a substrate,
- anodic electropolymerizing one electropolymerizable monomer or a plurality electropolymerizable monomers onto the conducting layer in the presence of an analyte to form a sensor film including analyte releasable monomer complexes, wherein the analyte is selected from the group consisting of a molecular analyte, a drug, and a peptide, and
- removing the analyte from the releasable complexes in the sensor film via an electrochemically mediated wash to form a sensor film including complimentary template-shaped cavities,
- wherein each of the monomers includes a terthiophene (3T) electropolymerizable group or a carbazole (CBz) electropolymerizable group and a CH2COOH pendant group, a CH2CH2OH pendant group, a CH2OH pendant group, or a CH2NH2 pendant group and are selected from the group consisting of 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)acetic acid (G0-3TCH2COOH), 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethanol (G0-3TCH2CH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methanol (G1-3T CH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methamine (G1-3T CH2NH2), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methanol (G1-CBzCH2OH), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methamine (G1-CBzCH2NH2), and mixtures thereof.
1 more element
A method comprising:
- forming a conducting layer on a surface of a substrate,
- anodic electropolymerizing one electropolymerizable monomer or a plurality electropolymerizable monomers onto the conducting layer in the presence of an analyte to form a sensor film including analyte releasable monomer complexes, wherein the analyte is selected from the group consisting of drug, and a peptide,
- removing the analyte from the releasable complexes in the sensor film via an electrochemically mediated wash to form a sensor film including complimentary template-shaped cavities,
- injecting a template onto the sensor film, wherein the film is situated in an electrochemical Surface Plasmon Resonance (SPR) apparatus or an electrochemical Quartz Crystal Microbalance (QCM) apparatus, and
- measuring a response to the injected template using (a) SPR spectroscopy or (b) QCM sensmg,
- wherein each of the monomers includes a terthiophene electropolymerizable group or a carbazole electropolymerizable group and a CH2COOH pendant group, a CH2CH2OH pendant group, a CH2OH pendant group, or a CH2NH2 pendant group and are selected from the group consisting of 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)acetic acid (G0-3TCH2COOH), 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethanol (G0-3TCH2CH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methanol (G1-3TCH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methamine (G 1-3TCH2NH2), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methanol (G1-CBzCH2OH), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methamine (G1-CBzCH2NH2), and mixtures thereof.
3 more elements
A method comprising:
- forming a conducting layer on a surface of a substrate,
- depositing one type of templating particle or plurality of types of templating particles on the conducting layer of the substrate to influence a morphology of the sensor film, where the particles improve analyte binding efficiency,
- anodic electropolymerization one electropolymerizable monomer or a plurality of electropolymerizable monomers onto the conducting layer in the presence of an analyte to form a sensor film including analyte releasable monomer complexes, wherein the analyte is selected from the group consisting of a molecular analyte, a drug, and a peptide, wherein the substrate from forming and depositing step is situated in an electrochemical Surface Plasmon Resonance (SPR) apparatus or an electrochemical Quartz Crystal Microbalance (QCM) apparatus,
- removing the analyte from the releasable complexes in the sensor film via an electrochemically mediated wash to form a sensor film including complementary template-shaped cavities,
- injecting a template onto the sensor film, and
- measuring changes in properties of the sensor film using (a) SPR spectroscopy or (b) QCM sensing in response to the injected template, wherein the response is substantially linear,
- wherein each of the monomers includes a terthiophene (3T) electropolymerizable group or a carbazole (CBz) electropolymerizable group and a CH2COOH pendant group, a CH2CH2OH pendant group, a CH2OH pendant group, or a CH2NH2 pendant group and are selected from the group consisting of 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)acetic acid (G0-3TCH2COOH), 2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethanol (G0-3T CH2CH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methanol (G1-3T CH2OH), 3,5-bis(2-(2,5-di(thiophen-2-yl)thiophen-3-yl)ethoxy)phenyl)methamine (G1-3T CH2NH2), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methanol (G1-CBzCH2OH), 3,5-bis(4-(9H-carbazol-9-yl)butoxy)methamine (G1-CBzCH2NH2), and mixtures thereof.
4 more elements
Dependencies are inferred from the claim text.