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US 10,787,523 B2Granted
Nanoparticle-protein complex for intracellular protein delivery
AssigneeUniversity of Massachusetts
InventorsVincent M. Rotello, Rubul Mout
Life of this patent
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Abstract
Various embodiments disclosed relate to a nanoparticle-protein complex for intracellular protein delivery. In various embodiments, the present invention provides a nanoparticle-protein complex including a nanoparticle including an amine-containing ligand. The nanoparticle-protein complex also includes a protein comprising a carboxylic acid-containing tag.
Claims19
A nanoparticle-protein complex comprising:
- a nanoparticle comprising an amine-containing ligand;
- a protein comprising a carboxylic acid-containing tag, wherein the amine-containing ligand and carboxylic acid-containing tag are joined by an electrostatic interaction to form the nanoparticle-protein complex;
- a nucleic acid material, having a largest dimension in a range of from about 0.5 nm to about 1000 nm and a molar ratio of protein to nucleic acid is at least about 1:1, wherein
- the nanoparticle-protein complex has a largest dimension of about 50 nm to about 999 nm.
1 more element
A nanoparticle-protein complex comprising:
- a gold nanoparticle comprising an amine-containing ligand having the structure:
- or a salt thereof, wherein m is 0 to 1,000 and n is 0 to 1,000;
- a protein comprising a carboxylic acid-containing tag having the structure:
- or a salt thereof,
- or,
- or a salt thereof,
- wherein n is about 1 to about 10,000, wherein the protein is chosen from GFP, Granzyme A, mCherry, Cre recombinase, Cas9, Histone 2A, and combinations thereof;
- and
- a nucleic acid material, having a largest dimension in a range of from about 0.5 nm to about 999 nm and a molar ratio of protein to nucleic acid is at least about 1:1,
- wherein the nanoparticle-protein complex has a largest dimension of about 50 to about 999 nm, the gold nanoparticle has a largest dimension of about 1 nm to about 50 nm, in solution the protein has a largest dimension of about 0.5 nm to about 50 nm, the gold nanoparticle has about 1 to about 10,000 of the amine-containing ligands, the nanoparticle-protein complex has about 1 to about 10,000 of the gold nanoparticles, and the nanoparticle-protein complex has about 1 to about 10,000 molecules of the protein and the amine-containing ligand and carboxylic acid-containing tag are joined by an electrostatic interaction to form the nanoparticle-protein complex.
7 more elements
A nanoparticle-protein complex comprising:
- a gold nanoparticle comprising an amine-containing ligand having the structure:
- or a salt thereof, wherein m is 0 to 1,000 and n is 0 to 1,000;
- a protein comprising a carboxylic acid-containing tag having the structure:
- or a salt thereof,
- or,
- or a salt thereof,
- wherein n is about 1 to about 10,000, wherein the protein is chosen from GFP, Granzyme A, mCherry, Cre recombinase, Cas9, Histone 2A, and combinations thereof;
- a nucleic acid material that is gRNA, having a largest dimension in a range of from about 0.5 nm to about 999 nm and a molar ratio of protein to nucleic acid is at least about 1:1;
- wherein the nanoparticle-protein complex has a largest dimension of about 50 to about 999 nm, the gold nanoparticle has a largest dimension of about 1 nm to about 50 nm, in solution the protein has a largest dimension of about 0.5 nm to about 50 nm, the gold nanoparticle has about 1 to about 10,000 of the amine-containing ligands, the nanoparticle-protein complex has about 1 to about 10,000 of the gold nanoparticles, the nanoparticle-protein complex has about 1 to about 10,000 molecules of the protein, and the nanoparticle-protein complex has about 1 to about 10,000 molecules of the nucleic acid material and the amine-containing ligand and carboxylic acid -containing tag are joined by an electrostatic interaction to form the nanoparticle-protein complex.
6 more elements
Dependencies are inferred from the claim text.