Useful microorganism and method for producing substance of interest
AssigneeKao Corporation
InventorsKougo Iida, Takumi Iwasaki, Tatsunari Nishi
Life of this patent
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Abstract
It is an object of the present invention to provide a bacterial strain that can decrease the amount of an intermediate Compound P converted into Metabolite M and efficiently accumulate Compound P in a medium that is not supplemented with Metabolite M or the final product generated from Metabolite M. The present invention provides a prokaryotic organism having all features (a) to (d) as defined in the specification so as to accumulate Compound P by regulating expression level of Enzyme X that converts Compound P as an intermediate metabolite into Metabolite M in a biosynthetic pathway in which Metabolite M indispensable for the growth is produced from a carbon source.
Claims9
A genetically-modified prokaryotic organism comprising features (a) to (d) below:
- (a) activity of at least one enzyme necessary for the production of chorismic acid selected from 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase,
- phosphoenolpyruvic acid synthetase, phosphoenolpyruvic acid carboxykinase, transketolase, and transaldolase;
- is enhanced compared with that of a corresponding wild-type organism;
- (b) activity of a first chorismic acid mutase is lost or reduced by mutation to a translation region or promoter of the gene encoding the first chorismic acid mutase compared with that of a corresponding wild-type organism;
- (c) a van A, rhcH, or pcaI gene promoter is functionally linked to a gene encoding a second chorismic acid mutase, and
- (d) a benA gene promoter or a nagI gene promoter is functionally linked to a gene encoding vanR, rhcR, or pcaR.
Dependencies are inferred from the claim text.