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US 10,781,437 B2Granted
D-stereospecific aminoacyl-tRNA synthetase and method of producing D-stereospecific aminoacyl-tRNA synthetase
AssigneeBoard of Supervisors of Louisiana State University
InventorsEric Allen First, Charles Joseph Richardson
Life of this patent
Estimating the term…
Abstract
A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.
Claims13
A method for increasing D-stereospecificity of an enzyme comprising:
- introducing a stereospecific editing domain into the enzyme;
- wherein the enzyme is Geobacillus stearothermophilus tyrosyl-tRNA synthetase;
- and
- the stereospecific editing domain is amino acids 83-275 of a Pyrococcus horikoshii phenylalanine-tRNA synthetase β-subunit of Seq. ID. No. 1, that includes one of,
- L202A,
- L210A,
- T221A,
- T236A,
- F125A/S211A,
- L202A/S211A,
- T221A/S211A,
- F125A/S202A/S211A,
- F125A/T221A/S211A,
- L202A/T221A/S211A,
- F145A/L202A/T221A/S211A, and
- L202A/T221A/S211A/T236A
- mutations, the mutations being between one and four variants;
- and
- the steriospecific editing domain being inserted between G161 and I162 on the Geobacillus stearothermophilus tyrosyl-tRNA synthetase of Seq. ID. No. 2.
16 more elements
A method for increasing D—stereospecificity of an enzyme comprising:
- introducing a stereospecific editing domain into the enzyme between adjacent glycine and isoleucine amino acids;
- wherein
- the enzyme is Geobacillus stearothermophilus tyrosyl-tRNA synthetase;
- the steriospecific editing domain is amino acids 83-275 of a Pyrococcus horikoshii phenylalanyl—tRNA synthetase β-subunit of Seq. ID. No. 1, having the substitutions F125A and S211A;
- the steriospecific editing domain being inserted between G161 and I162 on the Geobacillus stearothermophilus tyrosyl-tRNA synthetase of Seq. ID. No. 2;
- the enzyme causes at least one D-amino acid being used in place of a glycine residue in a translation process;
- the enzyme causes at least one D-amino acid to occupy an inverse conformational space in a translation process.
4 more elements
Dependencies are inferred from the claim text.