Biopesticide
AssigneesRhodes University, Citrus Research International (Pty) Ltd, River Bioscience (Pty) Ltd
InventorsSean Moore, Martin Hill, Caroline Knox, Tamryn Marsberg, Michael Jukes, Boguslaw Szewczyk, Lukasz Rabalski, Craig Chambers
Life of this patent
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Abstract
A nucleopolyhedrovirus (NPV), a composition comprising the NPV, and a method comprising the use of the NPV is provided. The NPV was isolated from Cryptophlebia peltastica and has insecticidal activity against several species of moths within the tortricid tribe, Grapholitini. The NPV or composition may be suitable for use in controlling insect populations, particularly populations of insects that infest plants.
Claims20
A biopesticidal composition for use in controlling insect populations, the composition comprising:
- a homogenized mixture of:
- larvae infected with a nucleopolyhedrovirus (NPV) having a genome sequence with at least 98% sequence identity to SEQ ID NO:
- 2;
- an artificial larval diet on which the larvae were reared;
- and
- occlusion bodies of the NPV from the infected larvae;
- water;
- and
- an additive which improves the stability or maintains the activity of the virus in the composition;
- wherein prior to being homogenized, the larvae have been cultivated, fed on the artificial diet, inoculated with an inoculum of the NPV, maintained on the artificial diet to allow the virus to replicate within the larvae, and harvested.
A method of controlling insect populations, the method comprising applying to the insects or their locus an insecticidally effective amount of a biopesticidal composition comprising:
- a homogenized mixture of:
- larvae infected with a nucleopolyhedrovirus (NPV) having a genome sequence with at least 98% sequence identity to SEQ ID NO:
- 2;
- an artificial larval diet on which the larvae were reared;
- and
- occlusion bodies of the NPV from the infected larvae;
- water;
- and
- an additive which improves the stability or maintains the activity of the virus in the composition;
- wherein prior to being homogenized, the larvae have been cultivated, fed on the artificial diet, inoculated with an inoculum of the NPV, maintained on the artificial diet to allow the virus to replicate within the larvae, and harvested.
A method of treating or preventing insect infestations on plants or fruit, the method comprising applying to the plants or fruit an insecticidally effective amount of a biopesticidal composition comprising:
- a homogenized mixture of:
- larvae infected with a nucleopolyhedrovirus (NPV) having a genome sequence with at least 98% sequence identity to SEQ ID NO:
- 2;
- an artificial larval diet on which the larvae were reared;
- and
- occlusion bodies of the NPV from the infected larvae;
- water;
- and
- an additive which improves the stability or maintains the activity of the virus in the composition;
- wherein prior to being homogenized, the larvae have been cultivated, fed on the artificial diet, inoculated with an inoculum of the NPV, maintained on the artificial diet to allow the virus to replicate within the larvae, and harvested.
Dependencies are inferred from the claim text.