Fluidic systems for analyses
AssigneeOPKO Diagnostics, LLC
InventorsVincent Linder, David Steinmiller, Samuel K. Sia
Life of this patent
Estimating the term…
Abstract
Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
Claims29
A solid-phase assay system comprising:
- a rigid substrate, having two or more reaction areas that are not connected with each other, each reaction area having at least one detection zone, wherein one or more of the detection zones contains a reagent immobilized on a rigid surface of the detection zone, wherein the two or more reaction areas are formed in the rigid substrate, and wherein contacting a sample to the detection zones allows binding between one or more components of the sample and one or more of the immobilized reagents;
- and
- one or more optical detectors aligned with the detection zones and connected to the electronics of a reader configured to detect and report an optical signal related to the binding of the one or more components of the sample to the one or more immobilized reagents on the detection zones.
A solid-phase assay system comprising:
- a first rigid substrate having one or more detection zones, wherein the one or more detection zones contains a nucleic acid reagent immobilized on the surface of the one or more detection zones, and wherein contacting a sample to the one or more detection zones allows binding between one or more nucleic acids in the sample and one or more of the nucleic acid reagents;
- a first optical detector aligned with one or more of the detection zones on the first rigid substrate and connected to the electronics of a first reader configured to detect and report a first optical signal related to the binding of one or more nucleic acids to the one or more nucleic acid reagents on the detection zones;
- a second rigid substrate having two or more reaction areas that are not connected with each other, each reaction area having at least one detection zone, wherein one or more of the detection zones contains a protein reagent immobilized on a rigid surface of the detection zone, wherein the two or more reaction areas are formed in the second rigid substrate, and wherein contacting a sample to the one or more detection zones allows binding between one or more proteins in the sample and one or more of the protein reagents;
- and
- a second optical detector aligned with one or more of the detection zones on the second rigid substrate and connected to the electronics of a second reader configured to detect and report a second optical signal related to the binding of one or more proteins to the one or more protein reagents on the detection zones.
Dependencies are inferred from the claim text.