Increasing productivity of E. coli host cells that functionally express P450 enzymes
AssigneeManus Bio Inc.
InventorsJason Donald, Christopher Pirie, Liwei Li, Huey-Ming Mak, Srishti Tibrewala, Ajikumar Parayil Kumaran
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Abstract
The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
Claims20
A method for biosynthesis of one or more chemical species in Escherichia coli, said method comprising:
- expressing one or more recombinant biosynthetic pathways that produce one or more chemical species in Escherichia coli, the one or more biosynthetic pathways comprising at least one membrane-anchored P450 enzyme, wherein at least a portion of the transmembrane region of the P450 enzyme is substituted with a transmembrane domain derived from an Escherichia coli inner membrane cytoplasmic C-terminus protein, and
- culturing the Escherichia coli to produce the one or more chemical species from the one or more biosynthetic pathways.
An Escherichia coli host cell expressing one or more recombinant biosynthetic pathways that produce one or more chemical species, where the biosynthetic pathways comprise at least one membrane-anchored P450 protein, wherein at least a portion of the transmembrane region of the P450 enzyme is substituted with a transmembrane domain derived from an Escherichia coli inner membrane cytoplasmic C-terminus protein.
A plant P450 enzyme comprising an N-terminal truncation and a single-pass transmembrane region derived from an Escherichia coli inner membrane cytoplasmic C-terminus protein.
Dependencies are inferred from the claim text.