Enhanced zein reduction in transgenic corn seed
AssigneeMonsanto Technology LLC
InventorsMichael H. Luethy, Thomas M. Malvar, Shihshieh Huang
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Abstract
Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
Claims5
A maize plant with reduced expression of at least two zein target genes, the plant comprising a DNA construct comprising a promoter operably linked to in 5′ to 3′ order a first anti-sense-oriented DNA element directed to a first zein target gene, a second anti-sense-oriented DNA element directed to a second zein target gene, and at least one sense-oriented DNA element that is complementary to a 5′ portion of the anti-sense-oriented DNA element(s), wherein a portion of the 3′ end of the second anti-sense-oriented DNA element is not complementary either to itself or to the sense-oriented DNA element, wherein transcription of the DNA construct produces a loop and a double-stranded RNA segment, and wherein the double-stranded RNA segment comprises at least 21 nucleotides.
Dependencies are inferred from the claim text.