# US 10,768,184 B2 · Methods for increasing palatability of pet foodstuff

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- Number: US 10,768,184 B2
- Document ID: US10768184B2
- Status: Granted
- Kind: B2
- Application number: 16/536,088
- Filed: 2019-08-08
- Published: 2019-11-28
- Granted: 2020-09-08
- Earliest claimed: 2013-06-14
- Assignees: Mars, Incorporated
- Applicants: Mars, Incorporated
- Inventors: Scott Joseph McGrane; Andrew John Taylor; Matthew Ronald Gibbs; Richard Masten Fine; Boris Klebansky
- Web page: https://patentgrep.com/patent/US10768184B2

## CPC

- G01N 33/74: involving hormones {or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors}
- C07C 53/126: Acids containing more than four carbon atoms
- C07C 57/03: Monocarboxylic acids
- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C07K 14/70596: {Molecules with a "CD"-designation not provided for elsewhere}
- G01N 33/566: using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds}
- G16B 35/00: ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
- G16C 20/60: In silico combinatorial chemistry
- G16C 20/64: Screening of libraries
- C07K 2319/00: Fusion polypeptide
- G01N 2333/70596: Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
- G01N 2333/726: G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07C: ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16C: COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE

## Abstract

The present invention relates to a method of identifying a compound that binds to or modulates the activity of one or more polypeptides encoding one or more receptors that are involved in the detection and perception of fatty acids.

## Claims

### Claim 1

A method for increasing palatability of a pet foodstuff comprising:
a. contacting a polypeptide with a compound, wherein the polypeptide comprises CD36 comprising the amino acid sequence set forth in SEQ ID NO:3 or SEQ ID NO:7,
b. measuring the biological activity of the polypeptide in the absence and in the presence of the compound, and
c. admixing the compound or a composition comprising the compound with a pet foodstuff when there is a difference between the biological activity in the absence, compared to the presence of the compound.

### Claim 2 (depends on 1)

A method according to claim 1, wherein the method is an in vitro method.

### Claim 3 (depends on 1)

The method of claim 1, wherein the biological activity of the polypeptide is measured in a cell comprising the polypeptide.

### Claim 4 (depends on 2)

The method of claim 2, wherein the polypeptide is expressed in a cell.

### Claim 5 (depends on 4)

The method of claim 4, wherein the polypeptide is expressed by a vector.

### Claim 6 (depends on 4)

The method of claim 4, wherein the biological activity of the polypeptide is measured by monitoring a calcium concentration or a cGMP activity within the cell.

### Claim 7 (depends on 6)

The method of claim 6, wherein the calcium concentration is monitored by fluorescence detection or luminescence detection.

### Claim 8 (depends on 7)

The method of claim 7, wherein the fluorescence detection comprises a calcium sensitive fluorescent dye.

### Claim 9 (depends on 1)

The method of claim 1, further comprising testing the compound in an animal feeding test.

### Claim 10 (depends on 1)

The method of claim 1, wherein the compound is present at a concentration of between 0.001% and 1% in the pet foodstuff.

### Claim 11 (depends on 1)

The method of claim 1, wherein the compound is present at a concentration of between 0.01% and 1% in the pet foodstuff.