# US 10,768,181 B2 · Detection of an antibody against a pathogen

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- Number: US 10,768,181 B2
- Document ID: US10768181B2
- Status: Granted
- Kind: B2
- Application number: 15/105,722
- Filed: 2014-12-17
- Published: 2016-11-03
- Granted: 2020-09-08
- Earliest claimed: 2013-12-17
- Assignees: The Brigham and Women's Hospital, Inc.
- Applicants: The Brigham and Women's Hospital, Inc.
- Inventors: Stephen J. Elledge; Harry B. Larman; Tomasz Kula; George Xu
- Web page: https://patentgrep.com/patent/US10768181B2

## CPC

- C40B 40/10: Libraries containing peptides or polypeptides, or derivatives thereof
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- G01N 33/56983: {Viruses}
- G01N 33/56994: {Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus}
- G01N 33/6854: {Immunoglobulins}
- G16B 25/00: ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B 25/20: Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
- C40B 30/04: by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- C40B 40/02: Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
- G01N 2469/20: Detection of antibodies in sample from host which are directed against antigens from microorganisms
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

## Abstract

Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof. One aspect provided herein relates to a method for detecting an antibody against a pathogen in a subject, the method comprising: (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library comprises a plurality of peptides derived from a plurality of pathogens, and (b) detecting a peptide bound to at least one antibody, thereby detecting an antibody capable of binding the peptide.

## Claims

### Claim 1

A method for detecting the presence of an antibody against a pathogen in a subject, the method comprising:
(a) contacting a reaction sample comprising a recombinant phage display library with a biological sample comprising antibodies, wherein the phage display library comprises a plurality of peptides derived from at least three unique pathogens
wherein the phage display library is constructed using a synthetic, programmable microarray from a starting library that is uniformly distributed and
(b) lysing the phage display library and amplifying DNA therefrom,
(c) detecting at least one antibody bound to at least one peptide expressed by the phage display library, thereby detecting the presence of an antibody against the at least one pathogen peptide in the biological sample.

### Claim 2 (depends on 1)

The method of claim 1, wherein the antibodies from the biological sample are first immobilized to a solid support adapted for binding IgM, IgA, or IgG subclasses.

### Claim 3 (depends on 1)

The method of claim 1, wherein the plurality of viral, fungal or bacterial peptides are each less than 300 amino acids long.

### Claim 4 (depends on 1)

The method of claim 1, wherein each peptide of the plurality of viral, fungal or bacterial peptides is expressed from a nucleic acid sequence and wherein the nucleic acid sequence comprises a common adapter region appended to an end of the nucleic acid sequence.

### Claim 5 (depends on 1)

The method of claim 1, wherein at least two antibodies are detected simultaneously.