Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AssigneeThe Trustees of Princeton University
InventorsRobert H. Austin, Zhaoning Yu, Jonas O. Tegenfeldt, Stephen Y. Chou, Han Cao
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Abstract
Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
Claims16
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one macromolecule is DNA and the detected signal is correlated to a base pair sequence of said DNA.
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one macromolecule is a restriction fragment length polymorphism.
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one macromolecule is at least one chromosome.
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one chromosome is analyzed to determine the presence of at least one single nucleotide polymorphism.
Dependencies are inferred from the claim text.