# US 10,768,113 B2 · Device for reading an IVD assay > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,768,113 B2 - Document ID: US10768113B2 - Status: Granted - Kind: B2 - Application number: 16/507,566 - Filed: 2019-07-10 - Published: 2020-01-09 - Granted: 2020-09-08 - Earliest claimed: 2015-10-15 - Assignees: Lumos Diagnostics IP PTY LTD - Applicants: Lumos Diagnostics IP PTY LTD - Inventors: William Samuel Hunter - Web page: https://patentgrep.com/patent/US10768113B2 ## CPC - G01N 21/6456: {Spatial resolved fluorescence measurements; Imaging} - G01N 21/274: {Calibration, base line adjustment, drift correction} - G01N 21/8483: {Investigating reagent band (test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04)} - G01N 33/53: Immunoassay; Biospecific binding assay; Materials therefor - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Embodiments generally relate to a system for reading fluorescent-labelled diagnostic assays for in-vitro diagnostic applications. The system comprises a receiving member adapted to receive a fluorescent-labelled diagnostic assay cartridge carrying a fluorescent-labelled diagnostic assay; at least one excitation module configured to illuminate the diagnostic assay, when the diagnostic assay cartridge is placed in the receiving member; a camera module for capturing an image of the illuminated diagnostic assay placed in the receiving member; a processor for receiving the captured image from the camera module and determining whether or not a target analyte was present in the diagnostic assay captured by the camera module; and memory storing firmware, the firmware including a brightness compensation module configured to adjust the intensity of an image of a diagnostic cartridge captured by the camera module, in order to emulate a uniform field of illumination over the diagnostic cartridge. The brightness compensation module is configured to adjust the intensity of the captured image based on an illumination compensation look-up table. ## Claims ### Claim 1 A system for reading diagnostic assays for in-vitro diagnostic applications, the system comprising: a receiving member that receives a diagnostic assay cartridge carrying a diagnostic assay; at least one excitation module that illuminates the diagnostic assay, when the diagnostic assay cartridge is placed in the receiving member; a camera module that captures an image of the illuminated diagnostic assay placed in the receiving member; a processor; and memory storing firmware accessible to the processor; wherein when the processor executes the firmware, the processor is caused to receive the captured image from the camera module, adjust the intensity of the image to emulate a uniform field of illumination over the diagnostic cartridge based on an illumination look-up table, and based on the captured image, determine an amount of a target analyte that was present in the diagnostic assay. ### Claim 2 (depends on 1) The system of claim 1, wherein the diagnostic assay cartridge is a fluorescent-labelled diagnostic assay cartridge carrying a fluorescent-labelled diagnostic assay, and the calibration marker is a fluorescent calibration marker. ### Claim 3 (depends on 1) The system of claim 1, further comprising at least one calibration marker located in the field of view of the camera module, for evaluating the expected response from the at least one excitation module. ### Claim 4 (depends on 3) The system of claim 3, wherein the brightness compensation module is configured to adjust the intensity of the captured image based an evaluation of the expected response from the at least one excitation module determined based on the at least one calibration marker. ### Claim 5 (depends on 3) The system of claim 3, wherein the calibration marker is located on the receiving member. ### Claim 6 (depends on 1) The system of claim 1, wherein the processor is further configured to compare the amount of the target analyte detected with a predetermined threshold to determine whether or not a threshold amount of the analyte was present in the diagnostic assay. ### Claim 7 (depends on 1) The system of claim 1, wherein the firmware includes an exposure control module for adjusting the exposure of an image captured by the camera module. ### Claim 8 (depends on 1) The system of claim 1, wherein the at least one excitation module, the camera module and the processor are located on an optical read device. ### Claim 9 (depends on 1) The system of claim 1, wherein the at least one excitation module and the camera module are located on an optical read device, and the processor is located on an external device remote from the optical read device. ### Claim 10 (depends on 1) The system of claim 1, wherein the system is in communication with at least one external processing device. ### Claim 11 (depends on 1) The system of claim 1, wherein the camera module comprises a CMOS camera module. ### Claim 12 (depends on 1) The system of claim 1, wherein the parts of the system are configured to be substantially fixed relative to one another while the camera module captures an image of the diagnostic assay. ### Claim 13 (depends on 1) The system of claim 1, wherein the at least one excitation module comprises at least two excitation modules. ### Claim 14 (depends on 13) The system of claim 13, wherein the excitation modules are each counter-disposed at an oblique angle to the receiving member. ### Claim 15 (depends on 13) The system of claim 13, wherein each of the at least two excitation modules are configured to operate contemporaneously to illuminate the diagnostic assay, when the diagnostic assay is placed in the receiving member. ### Claim 16 (depends on 1) The system of claim 1, wherein the illumination compensation look-up table is generated based on a calibration image captured by the camera module, and is stored in the firmware. ### Claim 17 (depends on 1) The system of claim 1, wherein the firmware includes a colour decoding module for separating an image captured by the camera module into colour channels. ### Claim 18 (depends on 1) The system of claim 1, wherein each excitation module comprises at least one LED. ### Claim 19 (depends on 1) The system of claim 1, wherein each excitation module comprises at least one light shaping element to produce a near-collimated beam of excitation light. ### Claim 20 (depends on 1) The system of claim 1, wherein each excitation module comprises at least one optical filter.