# US 10,767,210 B2 · Fiber sampler for recovery of bioaerosols and particles > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,767,210 B2 - Document ID: US10767210B2 - Status: Granted - Kind: B2 - Application number: 16/455,067 - Filed: 2019-06-27 - Published: 2019-10-31 - Granted: 2020-09-08 - Earliest claimed: 2012-02-17 - Assignees: Research Triangle, Inc. - Applicants: Research Triangle Institute - Inventors: David S. Ensor; Howard Jerome Walls; Karin K. Foarde; Susanne Vera Hering; Steven Russel Spielman - Web page: https://patentgrep.com/patent/US10767210B2 ## CPC - C12Q 1/24: Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms - G01N 1/2202: {involving separation of sample components during sampling} - G01N 2001/2217: {using a liquid} - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract An aerosol collection system and method. The system includes a bio-aerosol delivery device configured to supply bioparticles in a gas stream, a moisture exchange device including a partition member coupled to the gas stream and configured to humidify or dehumidify the bioparticles in the gas stream, and an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles. The method includes delivering bioparticles in a gas stream, humidifying or dehumidifying the bioparticles in the gas stream by transport of water across a partition member and into a vapor phase of the gas stream, and collecting the bioparticles by a collection medium. ## Claims ### Claim 1 A method for collecting aerosols, comprising: humidifying or dehumidifying bioparticles in a first gas stream by transport of water vapor a) between an exterior side of a partition member and an interior side of the partition member and b) into a vapor phase of a second gas stream including the bioparticles; delivering the bioparticles into the second gas stream; controlling a pressure of the first gas stream on the exterior side of the partition member to adjust a relative humidity for the bioparticles in the second gas stream; and collecting the bioparticles by a collection medium. ### Claim 2 (depends on 1) The method of claim 1, wherein humidifying or dehumidifying the particles comprises maintaining a relative humidity greater than 5%, greater than 10%, greater than 15%, greater than 25%, or greater than 50%. ### Claim 3 (depends on 2) The method of claim 2, wherein humidifying or dehumidifying the particles comprises transporting the water across the partition member comprising at least one of: a copolymer of perfluoro-3,6-dioxa-4-methyl-7octene-sulfonic acid and tetrafluoroethylene; polyethylene oxide, polyvinyl alcohol, cellulose ether and starch) and copolymers thereof; and polyacrylic acid, polyacrylamide, polyisopropyl acrylamide, polystyrene sulfonic acid, polyvinyl pyridine and polyamino acid and copolymers and salts thereof. ### Claim 4 (depends on 1) The method of claim 1, wherein humidifying or dehumidifying the particles comprises transporting the water vapor across the partition member comprising at least one of a permeable material, a semi-permeable membrane material, or a polymeric ionomer. ### Claim 5 (depends on 1) The method of claim 1, further comprising: stabilizing at least one of a temperature and relative humidity of the aerosol collection medium prior to collecting the bioparticles. ### Claim 6 (depends on 1) The method of claim 1, further comprising: controlling a temperature and a relative humidity along a gas-flow axis of the membrane. ### Claim 7 (depends on 6) The method of claim 6, further comprising: adjusting the temperature and the relative humidity such that the particles in the second gas stream transitions from outside ambient conditions to a target temperature and relative humidity condition. ### Claim 8 (depends on 7) The method of claim 7, further comprising: adjusting the temperature and the relative humidity to at least one of: 2° C. to 10° C. and RH of 5 to 95% 2° C. to 10° C. and RH of 70 to 85%; 1° C. to 8° C. and RH of 5 to 95%; 1° C. to 8° C. and RH of 70 to 85%; 2° C. to 6° C. and RH of 5 to 95%; 2° C. to 6° C. and RH of 70 to 85%; 4° C. and RH of 5 to 95%; or 4° C. and RH of 70 to 85%. ### Claim 9 (depends on 7) The method of claim 7, further comprising: adjusting a temperature and a relative humidity of the bioparticle while transitioning from outside ambient conditions to the target temperature and relative humidity condition along a controlled path of temperature and humidity, said controlled path comprises a two-stage system including a first stage having a relative humidity in a range from 40-60% at ambient temperature and a second stage below ambient temperature while maintaining the relative humidity in said range from 40-60%. ### Claim 10 (depends on 1) The method of claim 1, further comprising: providing a viability enhancing material to the collection medium. ### Claim 11 (depends on 1) The method of claim 1, wherein providing a viability enhancing material comprises providing at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth to the collection medium. ### Claim 12 (depends on 1) The method of claim 1, wherein collecting the bioparticles comprises collecting at least one of pox viruses, filoviruses, arenaviruses, alphavirus, Brucella species, Burkholderia mallei, Yersinia pestis and Coxiella burnetii on the collection medium. ### Claim 13 (depends on 1) The method of claim 1, wherein the partition member prevents contamination of the bioparticles in the second gas stream from contaminants in the first gas stream. ### Claim 14 (depends on 1) The method of claim 1, wherein the partition member comprises a tube in shell device preventing contamination.